Protocol for biomimetic tumoroid models by plastic compression using centrifugation

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Devereaux, Sam; Lam, Ashley; Upadhyay, Anuja; Fallows, Megan; Qiu, Lianqi; Pan, Xueni and Cheema, Umber. 2025. Protocol for biomimetic tumoroid models by plastic compression using centrifugation. STAR Protocols, 6(2), 103718. ISSN 2666-1667 [Article]

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Abstract or Description

Here, we present a protocol for engineering biomimetic tumoroid models by plastic compression using centrifugation. We describe steps for generating multi-compartment tumor-stroma models by mixing cells into a collagen hydrogel crosslinked at 37°C and centrifuging the hydrogel. We then detail procedures for generating compartmentalized models and encapsulating the final layered hydrogel containing a 96-well tumor mass in a 24-well stroma. This protocol increases collagen density and improves mechanical properties of collagen hydrogels.

Item Type:

Article

Identification Number (DOI):

https://doi.org/10.1016/j.xpro.2025.103718

Additional Information:

Funding: The authors would like to give thanks to their funding bodies: the UKRI MRC (MR/W006774/1) (S.D.) and UCB Pharma (A.U.). The graphical abstract was created in BioRender (Devereaux, 2025, https://BioRender.com/t65l774).

Data Access Statement:

This study did not generate any datasets/code.

Departments, Centres and Research Units:

Computing

Dates: